triton x 100 New England Biolabs Search Results


96
New England Biolabs triton x 100
Triton X 100, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs ligation buffer
Ligation Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs x taq dna ligase buffer
X Taq Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs 1x ecori buffer
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
1x Ecori Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
New England Biolabs bst dna polymerase
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs triton x 100 buffer
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Triton X 100 Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs tritonx 100
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Tritonx 100, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs nxs buffer
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Nxs Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs polysome lysis buffer
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Polysome Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad triton x 100
A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C <t>(EcoRI,</t> primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Triton X 100, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C (EcoRI, primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).

Journal: bioRxiv

Article Title: Human Papillomavirus Genomes Associate with Active Host Chromatin during Persistent Viral Infection

doi: 10.1101/2025.04.15.649012

Figure Lengend Snippet: A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C (EcoRI, primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).

Article Snippet: Nuclei were washed twice with 1X EcoRI buffer (NEB; 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl 2 , 0.025% Triton X-100, pH 7.5 at 25°C) to remove SDS and digested overnight with 400U EcoRI (NEB, R3101M) at 37°C, with shaking at 900 rpm.

Techniques: Sequencing, Inverse PCR, Control