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Image Search Results
Journal: bioRxiv
Article Title: Human Papillomavirus Genomes Associate with Active Host Chromatin during Persistent Viral Infection
doi: 10.1101/2025.04.15.649012
Figure Lengend Snippet: A, Schematic representation of the 4C-seq methodology. B, HPV31 genome showing restriction sites used for 4C (EcoRI, primary restriction enzyme; DpnII, secondary restriction enzyme). MboI, which shares the same recognition sequence as DpnII, was used for HiC. Inverse PCR primers (indicated by purple arrows) targeting three viewpoints (V1-V3) within the HPV31 genome were used for 4C analysis; the corresponding primary and secondary restriction sites targeted by the 4C primers are indicated by pink text. C, Heatmap showing Spearman rank correlation coefficients of 4C signals (average normalized reads across all four 9E samples) for 50 kb windows across the three viewpoints. D-F, Boxplots showing 4C signal intensities (average normalized reads across all four 9E samples for 50 kb windows) at HiC peaks relative to size-matched random control regions for viewpoints 1 (D), 2 (E) and 3 (F) on the HPV31 genome. Wilcoxon rank-sum test was used to determine statistical significance (****, p<0.0001).
Article Snippet: Nuclei were washed twice with
Techniques: Sequencing, Inverse PCR, Control